Optimization of DOTAP/DOPE/cholesterol/DMG-PEG 2000 lipid nanoparticles for delivery of E6-expressing mRNA as a therapeutic vaccine against HPV type 16

Title : Optimization of DOTAP/DOPE/cholesterol/DMG-PEG 2000 lipid nanoparticles for delivery of E6-expressing mRNA as a therapeutic vaccine against HPV type 16

Abstract:

Human Papillomavirus (HPV) type 16 is a leading cause of cervical cancer. While prophylactic vaccines exist, therapeutic options are limited. This study aims to optimize a Lipid Nanoparticle (LNP) formulation composed of DOTAP, DOPE, Cholesterol, and DMG-PEG 2000 for the delivery of E6-encoding mRNA. Various formulations with different lipid ratios were synthesized and characterized also different flow rate ratios were examined to achieve appropriate nanoparticle size and zeta potential . Cytotoxicity was evaluated using the MTT assay on HEK 293 cells at 24 and 48 hours. The results indicated that formulations with a 1:1 and 1:2 ratio of DOTAP/Cholestrol showed favorable biocompatibility compared to 2:1 ratios. This optimized LNP system holds promise as a potent delivery vehicle for therapeutic HPV vaccines.

Biography:

Fereshteh Moharamzadeh is a Ph.D. candidate in Nanotechnology from Sharif University of Technology, following her B.Sc. and M.Sc. in Materials Engineering from Sharif University and the University of Tehran, respectively. Her research sits at the intersection of material science and biology, focusing on microfluidic systems, gene therapy, and immunotherapy. Currently, she is exploring advanced nanotechnological approaches for targeted drug delivery.