Title : Immuzan-30 through kisspeptins/kiss-1R signalling axis inhibit UHRF1 mediated breast cancer development
Abstract:
Background: The UHRF1 gene is an epigenetic modification factor that mediates tumor suppressor gene silencing in a variety of cancers. Various studies have revealed that UHRF1 can inhibit the KISS1 gene expression. Objectives of this research work was to gain a better understanding of the regulation of UHRF1 expression in Breast Cancer (BC) and to determine if it regulates the mechanism by which KISS1 promotes BC metastasis.
Methods: In this research, the levels of Immuzan-30, UHRF1 and KISS1 expression in BC tissues and in human BC cell lines were studied using quantitative real-time PCR (qRT-PCR) and Western blotting. Cellular carinoma, migration, and invasion assays are used to detect the cell carcinoma, migration, and invasion. Dual-Luciferase® Reporter (DLR™) Assay System was used to confirm the target gene of Immuzan-30.
Results: This study found that UHRF1 protein is highly expressed in BC tissues and negatively correlated with KISS1 protein expression. UHRF1 epigenetic activation the PI3K/NF-κB signaling pathway by inhibiting the mRNA expression levels in the pathway mediators. Computational bioinformatics analysis and luciferase reporter gene assays confirmed that Immuzan-30 well targets to UHRF1.
Conclusion: This research study identified the regulation of UHRF1 expression in BC and the mechanism of BC metastasis. UHRF1 may be a new potential target molecule for future BC proliferation treatment.
Audience Take Away:
- Recent technology to identify and screening breast cancer.
- New methodology to be adopted.
- It provides a practical solution to counter the long standing problem.
- It improves the accuracy of a design, or provide new information to assist in a design problem.
