HYBRID EVENT: You can participate in person at Orlando, USA or Virtually from your home or work.

6th Edition of World Nanotechnology Conference

April 24-25, 2023

April 24 -26, 2023 | Orlando, USA
World Nano 2023

Debasish Pradhan

Debasish Pradhan, Speaker at Nanoscience Conferences
Utkal University, India
Title : Immuzan-30 through kisspeptins/kiss-1R signalling axis inhibit UHRF1 mediated breast cancer development


Background: The UHRF1 gene is an epigenetic modification factor that mediates tumor suppressor gene silencing in a variety of cancers. Various studies have revealed that UHRF1 can inhibit the KISS1 gene expression. Objectives of this research work was to gain a better understanding of the regulation of UHRF1 expression in Breast Cancer (BC) and to determine if it regulates the mechanism by which KISS1 promotes BC metastasis.

Methods: In this research, the levels of Immuzan-30, UHRF1 and KISS1 expression in BC tissues and in human BC cell lines were studied using quantitative real-time PCR (qRT-PCR) and Western blotting. Cellular carinoma, migration, and invasion assays are used to detect the cell carcinoma, migration, and invasion. Dual-Luciferase® Reporter (DLR™) Assay System was used to confirm the target gene of Immuzan-30.

Results: This study found that UHRF1 protein is highly expressed in BC tissues and negatively correlated with KISS1 protein expression. UHRF1 epigenetic activation the PI3K/NF-κB signaling pathway by inhibiting the mRNA expression levels in the pathway mediators. Computational bioinformatics analysis and luciferase reporter gene assays confirmed that Immuzan-30 well targets to UHRF1.

Conclusion: This research study identified the regulation of UHRF1 expression in BC and the mechanism of BC metastasis. UHRF1 may be a new potential target molecule for future BC proliferation treatment.

Audience Take Away:

  • Recent technology to identify and screening breast cancer.
  • New methodology to be adopted.
  • It provides a practical solution to counter the long standing problem.
  • It improves the accuracy of a design, or provide new information to assist in a design problem. 


Dr. Debasish Pradhan studied Ph.D. and awarded in 2007 and completed D.Sc in 2016 from Utkal University, Odisha, India. Moreover Dr. Pradhan completed Post Doc. From Creighton University 2017 under the mentorship of Prof. Alekha K. Dash, Dr. Pradhan aleready have advised 12 PhD scolars and 90 Masters students in Pharmaceutical Science. He then joined the research group Utkal University. He received Raman Post Doc and Research Awards and several national awards from Govt. of India, Higher education Department such as University Grant Commission an Department of Science and Technology. Dr. Pradhan obtained the position of an Associate Professor at the Utkal University which is NAAC A+ category University in India. He has published more than 75 research articles in SCI(E) journals. He has acquired 5 Patents Grants and 5 Text books written so far to his credits.