HYBRID EVENT: You can participate in person at Orlando, USA or Virtually from your home or work.

6th Edition of World Nanotechnology Conference

April 24-25, 2023

April 24 -26, 2023 | Orlando, USA
World Nano 2023

Seyed Mostafa Hosseini

Seyed Mostafa Hosseini, Speaker at Nanomaterials Conference
Hamadan University of Medical Sciences, Iran (Islamic Republic of)
Title : Doxycycline-encapsulated solid lipid nanoparticles for the enhanced antibacterial potential to treat the chronic brucellosis and preventing its relapse: In vivo study


Background: Brucellosis is one of the most important infection of diseases. Due to its large period of treatment and survival ability of bacteria inside the macrophages, relapse of this disease is the main challenge, especially, after the treatment. The current study was carried out to evaluate the antibacterial efect of solid lipid nanoparticles loaded with doxycycline on the Brucella melitensis in in vivo conditions.

Methods: The double emulsion synthesized doxycycline-encapsulated solid lipid nanoparticles (DOX-SLN) was characterized using DLS and FE-SEM. The efcacy of the DOX-SLN on the acute and chronic Wistar rat infected brucellosis was investigated. The pathological assessments were made on the spleen and liver in the treated rates.

Results: The in vivo experimental results demonstrated that the treated rats with DOX-SLN had significantly decreased the B. melitensis CFUs in their spleen and liver compared to that of the treated rates with free doxycycline and untreated ones. The pathologic results indicate that the improvement trend of spleen and liver tissues in rats treated by DOX-SLN was satisfactory.

Conclusion: According to in vivo results, the DOX-SLN has better efects on the treatment of chronic brucellosis. Therefore, DOX-SLN is recommended to treat the brucellosis and avoid its relapse.

Audience take away:

  • Synthesis method of solid lipid nanoparticles and drug loading method.
  • Investigating the properties of synthesized nanoparticles.
  • How the synthesized nanoparticle effects on bacteria in the cell line.


Seyed Mostafa Hosseini completed PhD degree in the field of medical bacteriology in the Hamadan University of Medical sciences in July 2019. His research interest is related to nanomedicine, nanoparticles, novel drug delivery systems and also performing molecular studies on bacteria (like intracellular or anaerobic bacteria). He has rich experiences in preparation and synthesis of nanoparticles for drug delivery, carrying out in vitro studies with different types of bacteria (specially intracellular and fastidious species such as Brucella and Mycobacterium), animal model handling and also cell culture.