Title : Antibacterial and anti-quorum sensing activity of PAMAM G4 loaded quorum quencher peptide against MRSA, staphylococcus aureus and AGR mutants
Methicillin-resistant Staphylococcus aureus (MRSA) is a cause of considerable concern in healthcare and the broader community. Globally, MRSA strains cause numerous infections with high morbidity and mortality rates. The success of MRSA is a result of the extensive production of virulence factors. With limited treatment options for MRSA infection and growing resistance problems, there is an urgent call to search for an alternative to antibiotics. One of the more promising strategies is targeting the quorum-sensing (QS) system of MRSA by using a dendrimer loaded with a kinase inhibitor peptide. This study will formulate poly(amidoamine) (PAMAM) dendrimers loaded with selected histidine kinase (HK) inhibitors. The physicochemical properties of the formula will be characterised using different in vitro analysis methods, such as light scattering and transmission electron microscopy (TEM). The antibacterial activity of the formula will be tested against MRSA and different mutant strains using a spot test, growth assay, and minimal inhibitory concentrations (MICs). Then, the anti-virulence effect of selected HK inhibitors will be evaluated using different mutant strains by haemolysis, biofilm, and kinase assays. Overall, the study will add to our knowledge of MRSA virulence factor expression and illuminate a potential strategy to inhibit those factors using an anti-virulence agent.
Audience Take Away:
- Treating MRSA infection is a huge challenge; the study will help in developing a new strategy for treating MRSA strains with a new anti-virulence agent using nanotechnology
- Enlighten about treating infection using none antibiotic option to reduce the resistance
- Studying the mechanism involved in anti-virulence effect of selected HK inhibitors as potential treatment option of bacteria infection