Title : Genotoxicity of noscapine nanosuspension prepared by microfluidic reactors on hepg2 cell line
Abstract:
Noscapine is an antispasmodic alkaloid used as antitussive and anti-cough obtained from plants about Papaveraceae family. This benzylisoquinoline alkaloid and its synthetic subsidiaries, called noscapinoids are being assessed for their anticancer potential. The present research aimed to investigate induction of DNA destruction and viability of HepG2 tumor spheroid culture influenced by noscapine and nanosuspention of noscapine. Culture of HepG2 cells as spheroids were treated with different concentrations of noscapine for 24 h on Day 11. Afterwards, viability assay and alkaline comet assay methods were applied to examine viability and induced DNA destruction, respectively. Based on the results, no significant impact was observed from Tween 40 on the viability and DNA damage levels in comparison with the control (p > 0.05). Moreover, increasing noscapine concentration resulted in a dose-dependent reduction in viability of hepatic cancer cells and elevation of DNA damages, showing a correlation between rises of DNA damages and viability decline.
Audience take away:
• Low solubility in water, or in other words, bioavailability is a common feature among the majority of commonly used chemotherapy drugs.
• Today, the most common method to increase the solubility of drugs is to use nanotechnology methods that reduce particle size to a sub-micron level.
• Production of particles with a small PDI is always one of the major problems in determining a method among common bottom-up and top-down methods.
• Microfluidic tools are considered the most sensitive, inexpensive and controllable method among bottom-up nano-tools.
