Unprecedented Redox scavenging signature alongwith antioxidant action of silver nanoparticle coupled with Andrographispaniculata(AP-Ag NP) against carbon tetrachloride (CCl4) induced toxicity in mice

Nano technology possesses several branches including nanomedicine, which is the most promising field in the future medicine and is a probable therapeutic agent in prevention and medication of life threatening diseases through ROS inhibition. Therapeutic potential and antioxidant activity of Silver Nanoparticle coupled with Andrographis paniculata (AP-Ag NP) was assessed against CCl₄ induced oxidative stress at tissue level. The main aim and objective of the study is to find out the comparative efficacy of AP-AgNP against carbon tetrachloride (CCl₄) induced oxidative stress model. Carbon tetrachloride (CCl₄) was administered upon Swiss albino mice (male) for 28 days concurrently with AP-AgNP (50 mg/kg body weight) orally to evaluate the therapeutic effects on hepatic oxidative injury, antioxidant potential and heme synthesis pathway. Serum ROS level was significantly elevated and blood and liver superoxide dismutase (SOD), catalase (CAT) activity and GSH level also significantly decreased after exposure of carbon tetrachloride (CCl₄). Treatment with AP-AgNP, as nano-antioxidant significantly increased SOD, CAT activity and GSH levels which indicate the recovery of oxidative injury and indicates restoring inhibited aminolevulinate dehydratase (ALAD) activity.  In conclusion our results suggest that Silver Nanoparticle synthesized using Andrographis paniculata (AP-Ag NP) have the potential antioxidant effect in experimental animals.