The aim of the present work was to prepare and characterization of atazanavir loaded nanostructured lipid carriers, Atazanavir (ATV), chosen is a BCS class II drug with poor water solubility and high permeability (log p of 4.11). In adults, ATV-recommended therapeutic dose is 400 mg once a day. Besides these, it undergoes rapid first-pass metabolism in liver, leading eventually to marked reduction in the drug oral bioavailable fraction (i.e., 60%) in humans and animals such as rats, and so on. To circumvent the aforementioned limitations, various formulation approaches of ATV have been reported such as, nanocrystals, nanoparticles, tablets and capsules but all with limited fruition. Atazanvair loaded nano structured NLC formulation was prepared by solvent emulsification-evaporation technique. The formulated solid lipid nanoparticles have the mean particle size 130 nm, zeta potential was found to be -13.2 mV and Polydispersity index values were found to be 0.27. DSC and FTIR studies revealed change in the crystallinity index of drug when incorporated into NLC. Cell lines studies and In-vitro cytotoxicity studies was carried out to evaluate the efficacy and safety of the formulation.
- They audience can learn about the method of preparation of atazanavir loaded nanostructured lipid carriers.
- Characterization technique’s involved in the study.
- Use of this drug loaded NLC in exhibiting the in vitro cytotoxicity studies using an appropriate cell lines.