Title : Callus mediated green synthesis of nanopartcles under controlled conditions and their antimicrobial activities
The present study was an attempt to develop a common laboratory procedure for synthesis of nanoparticles from callus of C. roseus under controlled conditions. The nanoparticles synthesized by the callus extract had proven to be an effective tool for synthesizing nanoparticles of small size (<100 nm) and uniformly distributed as confirmed by TEM, DLS and AFM analysis. In conclusion the study offers the potential method to synthesize new economically viable potential green particles having high efficacy, less cytotoxic, feasibility, and eco friendly in nature. The synthesized NPs demonstrated moderate antimicrobial activity against both Gram +ve and Gram-ve bacteria individually and in combination with antibiotics. The antibacterial activity of nanoparticles individually and in combination with existing antibiotics and natural products will encourage to check more new compounds and also providing a new route against multi drug resistant bacteria. The synergistic effect also increase the efficiency of natural compounds at very low concentration that will help to treat more and more patients effectively. The green particles may decrease the dependency on synthetic drugs and also protect patient from the painful traditional methods of treatment.
- Present work was focused on synthesis of green nanoparticles from callus under controlled conditions.
- This work will help to researchers to generate the callus by using different physiological conditions and photoperiod. Changes in physiological and photoperiod conditions are very important factors to increase the production of secondary metabolites which were act as reducing agents in synthesis of green nanoparticles. This work will also help to regulate the size and shape of synthesize nanoparticles.