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4th Edition of World Nanotechnology Conference

April 25-27, 2022

April 25 -27, 2022 | Las Vegas, USA
2022 Speakers

Nano-structured Fe3O4 (Magnetite) Powder Synthesis using Co-Precipitation Method for Bio-separation Applications

Mert Saracoglu, Speaker at
İstanbul Technical University , Turkey
Title : Nano-structured Fe3O4 (Magnetite) Powder Synthesis using Co-Precipitation Method for Bio-separation Applications

Abstract:

Magnetic separation is one of the most efficient and mild isolation technique for many biomedical applications such as gene therapy and clinical diagnosis where alternatives could harm the target molecule. Herein this work, superparamagnetic Fe3O4 powders were synthesized by co-precipitation method and characterized for its potential applications in DNA extraction & purification. Proposed method was optimized by adjusting the pH and the stabilizer concentration. As a result, aggregated Fe3O4 nanoparticles having high phase purity and superparamagnetic property was achieved. Formed phases were investigated using X-ray diffraction (XRD). Besides, purity of the phases was determined by Rietveld Analysis. At pH of 12.5 maximum %94 magnetite formation was achieved. Morphological analysis and particle size distribution of the synthesized powders were done by both scanning electron microscope (SEM) and Scherrer Formula. Results have suggested that the synthesized particles were in the size of micrometer which made up from nanoparticles between 50 - 110 nm in size. Magnetic property of Fe3O4 were measured using vibrating sample magnetometer (VSM). According to results, Fe3O4 particles have shown superparamagnetic behaviour, in the value of 40 emu/g. where superparamagnetic ability arises from the nanosized crystallites and nanoparticles present in the structure. Comprehensively, Co-precipitation of Fe3O4 particles with proposed pH and PVP concentration, offers easy and non-toxic powder synthesis with a short reaction time which is believed to enable industrial scale Fe3O4 powder production. Fe3O4 particles, produced with this method, could widen the use of magnetic separation in DNA purification and extraction. As a result, allowing the save time and resources

Biography:

Mert Saraçoğlu studied Material and Metallurgy Engineering at the İstanbul Technical University, Turkey and graduated in 2021. Currently applied for MSc degree in the same university, the research group of Prof. Servet Timur. Where he worked for two and half year in the topic of nano & micro particle synthesis.

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