HYBRID EVENT: You can participate in person at Orlando, USA or Virtually from your home or work.

6th Edition of World Nanotechnology Conference

April 24-25, 2023

April 24 -26, 2023 | Orlando, USA
World Nano 2023

Srivalli Mukkavilli

Srivalli Mukkavilli, Speaker at Nanotechnology Conferences
North Carolina Central University, United States
Title : Effects of Particulate Matter-Induced Reactive Oxygen Species and Carbon nanodots on Amyloid Beta Protein Aggregation: Relevance in Neurodegenerative Diseases


Alzheimer’s Disease is one of the most common neurodegenerative illness with multiple etiological mechanisms explored.  Accumulation of different sizes of beta amyloid (βA) proteins generated from Amyloid Protein Precursors (APPs) is known to induce a plaque formation. In a typical beta-amyloidogenic APP metabolism, about 90 percent of βA proteins are those with 40-amino acids while 42-amino acids make up the rest.  Initiation of the plaque formation or aggregation process of related proteins can be induced by various mechanisms.  Regardless, the presence of Reactive Oxygen Species (ROS) and free radicals is known to be one of the pathological mechanisms like other common forms of diseases.  As a source for ROS and free radicals, we have tested a potential role of the particulate matter (PM) collected from residential indoor settings in the aggregation of the lyophilized βA proteins.  PM is well known as an inducer of inflammatory process in many biological organisms, and ROS and free radicals from the PM are believed to be the causal agents. In our study, lyophilized forms of βA proteins were used to test their aggregation properties in the presence of indoor PM extracts at different concentrations.  The same PM extracts were used as a source of ROS in the presence of anti-DMPO adduct antibodies for assessing the aggregation level of βA protein generated by IMR-32 cells.  Radicalized βA proteins were bound by DMPO nitron adduct which was identified by anti-DMPO antibody. Then secondary antibody was used for quantifying purpose through ELISA. The aggregation extent was also visualized by using Con-focal imaging.  Results indicated that: 1) PM extracts showed a potential to induce aggregation of lyophilized βA proteins, 2) βA proteins aggregation is proportional to the level of PM exposed, 3) Aggregation extent of the βA proteins in In Vitro settings was consistent with the case of lyophilized proteins, 4) The formation of aggregates was confirmed by Con-focal imaging, and 5) Carbon nanodots have protective effects through ROS scavenging potential.  From the results, it is concluded that poor air quality induced by PM exposure, especially chronic exposure conditions, is a potential etiological mechanism of Alzheimer’s disease by inducing plaque formation in the brain tissues.  The level and the duration of exposure to PM before clinical symptoms start showing would be an area for investigation in the coming years.    


Srivalli Mukkavilli studied Pharmacy in India. She is currently working with a Dr. John Bang’s group as a second year graduate student in the Department of Pharmaceutical Sciences at North Carolina Central University.  She is currently working on her thesis focusing on the conformational effects on the level of βA aggregate formation by using an Electron Paramagnetic Resonance (EPR) based anti-DMPO adduct antibody detection method. From her study, she hopes to better understand the relative affinity of different βA proteins that can be used for finding a treatment method for AD.