HYBRID EVENT: You can participate in person at Orlando, USA or Virtually from your home or work.

6th Edition of World Nanotechnology Conference

April 24-25, 2023

April 24 -26, 2023 | Orlando, USA
World Nano 2023

Shubhangi Shukle

Shubhangi Shukle, Speaker at World Nanotechnology conferences
NCSU, United States
Title : Square wave voltammetric approach to leptin immunosensing and optimization of driving parameters with chemometrics


Square wave voltammetry serves as an effective analytical means to evaluate antigen-antibody coupling at the solid-liquid interface. Herein, we describe 3- aminopropyltrimethoxysilane (APTMS) induced irreversible immobilization of anti-leptin to micellar gold nanoparticles (AuNPs). Antibodies (Abs) were orthogonally loaded on micellized AuNP assemblies via amino residual groups. The ratio of bound Ab molecules was determined by the Bradford assay. The AuNP/Ab layer modified electrodes with variable antibody surface coverage (~400 ± 55–200 ± 30 Ab/NP) were analyzed in terms of change in backward, net current (Ip) components. The rate of antigen coupling was found to be consistent with the variation in antibody density as well as the binding affinity. The lowest detection limit was observed at the femtomolar level (0.25 fM/mL) over a wide range of antigen concentration (6.2 ng/mL to 0.12 fg/mL). The variables affecting the epitope-paratope interaction were further optimized using a chemometric approach and a response surface methodology (RSM).


Dr. Shubhangi earned her PhD in Analytical Chemistry at the IIT BHU, Varanasi India in 2019, and post graduated as MS in 2014, from University of Lucknow, India. She then joined the research group of Prof. Roger J Narayan at the Joint department of Biomedical engineering at North Carolina state University, as postdoctoral fellow. She has published more than 20 research articles in SCI(E) journals.