Title : Pentacyclic triterpenoid nano formulations as bioavailable drug delivery agents at cellular level
Abstract:
The last decade an increasing scientific interest was focused on pentacyclic triterpenoids (betulin, betulinic acid and lupane), considering their antitumor effects without so many secondary adverse actions comparative to other synthetic anticarcinogens (e.g. Doxorubicin). Considering that pentacyclic terpenoids have a low bioavailability, new nano formulations can overcome this challenge. Our aim was to find best solvents for these molecules and to obtain nano formulations (liposomes, nano lipid particles, selfassembled structures) from a standardized extract of Betula pendula bark, in order to build bioavailable agents for targeted delivery to normal vs tumour cells (e.g. Walker 256 cells). The nano formulations obtained were characterized by size DLS measurements, UV-VIS spectrometry, fluorescence microscopy and high-performance liquid chromatography coupled with mass spectrometry (LC_MS) and compared with similar formulations containing Doxorubicin. Comparatively, the viability tests and the apoptotic effects demonstrated that nano formulated triterpenoids can reach increased bioavailability and similar antitumor effects as Doxorubicin, but with less toxicity on normal cells.rubicin. Comparatively, the viability tests and the apoptotic effects demonstrated that nano formulated triterpenoids can reach increased bioavailability and similar antitumor effects as Doxorubicin, but with less toxicity on normal cells.
Audience Take Away:
- Knowledge about pentacyclic terpenoids and their natural sources, as well the recent data related to their impact on health and disease
- To see new innovative ways to make nanoformulations for future applicxations
- New ways to make these valuable compounds to be used, as nano formulations in vitro and in vivo, as anticancer agents
