Title : Harnessing Fe3O4 nano-sensor for detecting epinephrine in buff orpington rooster and rhodes island white broiler
This study presents findings resulting from the detection of epinephrine (EP) in real samples from chickens using Fe3O4-modified screen-printed silver electrodes (SPSE). The UV-visible spectroscopy, Fourier-Transform infrared spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), and Scanning electron microscopy (SEM) were used for characterizing iron oxide (Fe3O4) nanoparticles prior to the modification of the SPSE. Results show a corresponding rise in the oxidation peak current (Iap) of EP with an increase in EP’s concentration and the scan rate (25-400 mV s-1). Using square wave voltammetry (SWV), a detection limit of 19.3 μM (S/N=3) over a linear range of 9.99-60.9 µM was obtained. Simultaneous detection of EP in the presence of ascorbic acid was also obtained at this electrode. The fabricated sensor used to detect EP in the blood serum, breast muscle, kidneys, spleens, and livers of chicken species, namely Buff Orpington rooster (OR) and Rhodes Island White broiler (RIWB), produced better recovery.